HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hanks, M.C. Articles by Sang, H.M. Search for Related Content PubMed PubMed Citation Articles by Hanks, M.C. Articles by Sang, H.M.

A cDNA library was prepared from mRNA isolated from anterior pituitary glands of incubating bantam hens, in which prolactin mRNA levels were predicted to be very high. Nine clones, representing abundant mRNA species, were identified and shown to contain homologous sequences. Two clones, of 871 bp and 580 bp, were analysed by DNA sequencing. The shorter clone was found to be a truncated cDNA product but otherwise identical to the longer clone. The 871 bp cDNA, PRL101, contains an open reading frame capable of encoding a polypeptide of 229 amino acids. This putative polypeptide has a high degree of homology to mammalian prolactins (approximately 70%), strongly suggesting that PRL101 encodes chicken preprolactin. The protein was predicted to have a 30 amino acid signal sequence which would be cleaved off to give a mature protein of 199 amino acids. The peptide sequence also had a 26% homology to chicken growth hormone, which is related to prolactin. This similarity confirms the conclusion that PRL101 is a chicken prolactin cDNA clone. An abundant mRNA of approximately 880 b was detected in poly(A)⁺ RNA from pituitary glands probed with PRL101. Analysis of chicken genomic DNA showed that there is one copy of the prolactin gene in the genome. PRL101 hybridized strongly to genomic DNA from closely related galliforms (quail and turkey) and less strongly to DNA from more distantly related species (duck and ring dove).

This article has been cited by other articles:

				B. Leclerc, D. Zadworny, G. Bedecarrats, and U. Kuhnlein Ontogenesis of the Expression of Prolactin Receptor Messenger Ribonucleic Acid During Late Embryogenesis in Turkeys and Chickens Poult. Sci., June 1, 2007; 86(6): 1174 - 1179. [Abstract] [Full Text] [PDF]

				N. Nakao, T. Takagi, M. Iigo, T. Tsukamoto, S. Yasuo, T. Masuda, T. Yanagisawa, S. Ebihara, and T. Yoshimura Possible Involvement of Organic Anion Transporting Polypeptide 1c1 in the Photoperiodic Response of Gonads in Birds Endocrinology, March 1, 2006; 147(3): 1067 - 1073. [Abstract] [Full Text] [PDF]