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DOI: 10.1677/jme.0.0190267
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Journal of Molecular Endocrinology, Vol 19, Issue 3, 267-277
Copyright © 1997 by Society for Endocrinology

Articles

Cloning and characterization of the rat gene for the acid-labile subunit of the insulin-like growth factor binding protein complex

PJ Delhanty and RC Baxter


The acid-labile subunit (ALS) of the ternary insulin-like growth factor-binding protein complex has a central role in controlling the bioavailability of circulating insulin-like growth factors. We have shown that gene expression of ALS is regulated by a number of factors, particularly growth hormone. Our aim was to characterize the ALS gene in order to define the mechanism of its regulation. Southern analysis suggests a single copy of the ALS gene in the rat genome. The protein-coding and 3'-untranslated regions span approximately 3.5 kilobases of rat genome and are divided into two exons. The 5' flanking region of the gene lacks a consensus TATA-box or Inr sequence, and primer extension and reverse transcriptase PCR experiments locate multiple transcriptional initiation sites between -505 and -385 nucleotides relative to the translational initiation codon. This putative promoter region, when inserted upstream of the luciferase reporter gene, directs luciferase expression when transfected into H4-II-E cells. Our data demonstrate the uncomplicated structure of the rat ALS gene, and the promoter function and presence of potential regulatory elements in the region upstream of the protein-coding sequence.


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S.-E. Kong, S. M. Firth, R. C. Baxter, and P. J. D. Delhanty
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R. P. Rhoads, P. L. Greenwood, A. W. Bell, and Y. R. Boisclair
Organization and Regulation of the Gene Encoding the Sheep Acid-Labile Subunit of the 150-Kilodalton Insulin-Like Growth Factor-Binding Protein Complex
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A. Suwanichkul, Y. R. Boisclair, R. C. Olney, S. K. Durham, and D. R. Powell
Conservation of a Growth Hormone-Responsive Promoter Element in the Human and Mouse Acid-Labile Subunit Genes
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