| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The aim of this study was to establish the gestational- and labour-associated variation in the relative abundance of prostaglandin synthase-1 (PGHS-1) and prostaglandin synthase-2 (PGHS-2) mRNA in ovine placenta (cotyledons). Cotyledons were collected from non-labouring ewes at 40–145 days of gestation (n=25) and from ewes in active labour (145–147 days, n=5). The relative abundance of PGHS-1 and PGHS-2 mRNA transcripts was determined by Northern blot analysis and laser densitometry, using a 2·3 kb sheep and a 1·2kb mouse cDNA probe respectively. Data were expressed as a ratio of PGHS transcript hybridization/18S rRNA hybridization. During pregnancy, the relative abundance of PGHS-2 mRNA increased sevenfold, from 0·19±0·04 at 40–85 days (n=5) to 1·39±0·05 at 140–145 days (n=4) (P<0·01). PGHS-1 mRNA relative abundance did not change significantly (P>0·05) during gestation. Neither PGHS-1 nor PGHS-2 mRNA relative abundance changed significantly in association with labour onset at term (n=5) when compared with the relative abundance observed at 140–145 days (n=4) (P>0·05). The data obtained in this study are consistent with the hypothesis that PGHS-1 is constitutively expressed in ovine placenta during pregnancy and at the time of labour, and that PGHS-2 is induced during the second half of pregnancy. It remains to be established to what extent these two isozymes contribute to the net prostaglandin-forming capacity of the ovine placenta, particularly at the time of labour.
This article has been cited by other articles:
|
|
 |
|
  Q. Zhang and W. X. Wu Separate and synergistic effect of progesterone and estradiol on induction of annexin 2 and its interaction protein p11 in pregnant sheep myometrium J. Mol. Endocrinol., April 1, 2007; 38(4): 441 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhang, V. Collins, K. Chakrabarty, R. F. Wolf, N. Unno, D. Howe, J. C. Rose, and W. X. Wu Regulation of Membrane-Associated Prostaglandin E2 Synthase 1 in Pregnant Sheep Intrauterine Tissues by Glucocorticoid and Estradiol Endocrinology, August 1, 2006; 147(8): 3719 - 3726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. K. Palliser, J. J. Hirst, G. E. Rice, G. T. Ooi, N. L. Dellios, R. M. Escalona, and I. R. Young Labor-Associated Regulation of Prostaglandin E and F Synthesis and Action in the Ovine Amnion and Cervix Reproductive Sciences, January 1, 2006; 13(1): 19 - 24. [Abstract] [PDF]
|
|
|
|
|
|
W. X. Wu, X. H. Ma, T. Coksaygan, K. Chakrabarty, V. Collins, J. Rose, and P. W. Nathanielsz Prostaglandin Mediates Premature Delivery in Pregnant Sheep Induced by Estradiol at 121 Days of Gestational Age Endocrinology, March 1, 2004; 145(3): 1444 - 1452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Arosh, S. K. Banu, P. Chapdelaine, and M. A. Fortier Temporal and Tissue-Specific Expression of Prostaglandin Receptors EP2, EP3, EP4, FP, and Cyclooxygenases 1 and 2 in Uterus and Fetal Membranes during Bovine Pregnancy Endocrinology, January 1, 2004; 145(1): 407 - 417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.L. Martin, W.L. Whittle, A.C. Holloway, S. Gyomorey, W. Gibb, S. Lye, and J.R.G. Challis Ontogeny and Regulation of Ovine Placental Prostaglandin E2 Synthase Biol Reprod, September 1, 2002; 67(3): 868 - 873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. X. Wu, X. H. Ma, N. Unno, and P. W. Nathanielsz In Vivo Evidence for Stimulation of Placental, Myometrial, and Endometrial Prostaglandin G/H Synthase 2 by Fetal Cortisol Replacement after Fetal Adrenalectomy Endocrinology, September 1, 2001; 142(9): 3857 - 3864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R.G. Challis, S. G. Matthews, W. Gibb, and S. J. Lye Endocrine and Paracrine Regulation of Birth at Term and Preterm Endocr. Rev., October 1, 2000; 21(5): 514 - 550. [Abstract] [Full Text]
|
|
|
|
|
|
J. L. Cook, D. B. Zaragoza, D. H. Sung, and D. M. Olson Expression of Myometrial Activation and Stimulation Genes in a Mouse Model of Preterm Labor: Myometrial Activation, Stimulation, and Preterm Labor Endocrinology, May 1, 2000; 141(5): 1718 - 1728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gyomorey, S.J. Lye, W. Gibb, and J.R.G. Challis Fetal-to-Maternal Progression of Prostaglandin H2 Synthase-2 Expression in Ovine Intrauterine Tissues During the Course of Labor Biol Reprod, March 1, 2000; 62(3): 797 - 805. [Abstract] [Full Text]
|
|
|
|
|
|
G. Charpigny, P. Reinaud, J.-P. Tamby, C. Creminon, J. Martal, J. Maclouf, and M. Guillomot Expression of Cyclooxygenase-1 and -2 in Ovine Endometrium During the Estrous Cycle and Early Pregnancy Endocrinology, May 1, 1997; 138(5): 2163 - 2171. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
