HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Egerton, M Articles by Hollis, M Search for Related Content PubMed PubMed Citation Articles by Egerton, M Articles by Hollis, M

The human breast carcinoma cell line T47D is known to express high-affinity calcitonin receptors (CTRs). PCR amplification of the CTR cDNA from T47D mRNA resulted in the identification of two different cDNAs that encode distinct receptor isoforms, hCTR and hβCTR. The two cDNAs are identical except that the hCTR cDNA contains a 48 bp insert sequence that encodes a 16 amino acid domain in the first cytosolic loop of the receptor. Stable transfection of each receptor cDNA into murine erythroleukaemia (MEL) cells resulted in the expression of receptors with high affinity for radiolabelled salmon calcitonin (hCTR K_d 0·09 nM, hβCTR K_d 0·12 nM). Ligand competition binding studies did not reveal any significant pharmacological difference between the receptor isoforms. In transfected MEL cells and COS-1 cells the hβCTR isoform was expressed at tenfold higher levels than the hCTR. A reporter gene assay that monitored the coupling of CTR to adenylate cyclase by increases in β-galactosidase activity indicated that both receptors were able to stimulate cyclic AMP production in response to ligand binding.

This article has been cited by other articles:

				W.-J. Chen, S. Armour, J. Way, G. Chen, C. Watson, P. Irving, J. Cobb, S. Kadwell, K. Beaumont, T. Rimele, et al. Expression Cloning and Receptor Pharmacology of Human Calcitonin Receptors from MCF-7 Cells and Their Relationship to Amylin Receptors Mol. Pharmacol., December 1, 1997; 52(6): 1164 - 1175. [Abstract] [Full Text]

				J.-F. Shyu, D. Inoue, R. Baron, and W. C. Horne The Deletion of 14Amino Acids in the Seventh Transmembrane Domain of a Naturally Occurring Calcitonin Receptor Isoform Alters Ligand Binding and Selectively Abolishes Coupling to Phospholipase C J. Biol. Chem., December 6, 1996; 271(49): 31127 - 31134. [Abstract] [Full Text] [PDF]